4-HNE(4-Hydroxynonenal) ELISA Kit (E-EL-0128)
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For research use only.
Product Summary
| Sensitivity | 0.38 ng/mL |
| Detection Range | 0.63-40 ng/mL |
| Sample Volume | 50 μL |
| Total Assay Time | 2 h 30 min |
| Reactivity | Universal |
| Specificity | This kit recognizes Universal 4-HNE in samples.No significant cross-reactivity or interference between Universal 4-HNE and analogues was observed |
| Recovery | 80%-120% |
| Sample Type | Serum, plasma and other biological fluids |
| Detection Method | Colorimetric method, ELISA, Competitive |
| Assay Type | Competitive-ELISA |
| Size | 96T / 48T / 24T / 96T*5 / 96T*10 |
| Storage | 2-8℃ |
| Expiration Date | 12 months |
Performance
| Typical data |
The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only.
Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.
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| Precision |
Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.
Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.
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| Recovery |
The recovery of Universal 4-HNE was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.
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| Linearity |
Linearity of the assay was evaluated by spiking samples with high concentrations of Universal 4-HNE and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range.
The measured values were then compared to the expected concentrations to assess the linearity of response.
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| Stability |
Each kit batch is subjected to accelerated stability testing and real-time stability monitoring. Sample performance is evaluated after storage at 37 °C for 10 days to assess the impact of elevated temperature on assay reliability and reagent integrity.
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Test Principle
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal 4-HNE. During the reaction, Universal 4-HNE in the sample or standard competes with a fixed amount of Universal 4-HNE on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal 4-HNE. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal 4-HNE in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Background
An important link between elevated cytokines and oxidative stress during the initial inflammatory process changes the REDOX balance through a thiol-dependent mechanism, and HNE is the most active aldehydes produced during this process
| Research Area | Neuroscience , Cell Biology , Metabolism |
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