For research use only.
| Product Introduction |
5% Blocking Buffer is a common western blotting and immuno-histochemical Blocking Buffer, which is suitable for horseradish peroxidase (HRP), alkaline phosphatase (AP) and biotin labeled second antibody.
The preservatives in this product has not affect the activity of HRP and AP, and it will not interfere with the detection of HRP or AP labeled secondary antibody. At the same time, the product does not contain biotin and will not interfere with biotin based detection. This product is a Ready-to-Use product. It can be directly used to block the membrane and immunohistochemistry without any additional reagent. |
| Applications | WB, IHC |
| Storage | Store at 2~8°C for 12 months. Avoid of freezing. |
| Shipping | Biological ice pack at 4 ℃ |
| Cautions |
1. Usually we incubate with the Blocking Buffer at RT for 1 h. It can also be incubated at 4°C overnight. 2. There is no Blocking Buffer suitable for all experimental systems, for some special experiments, it may be necessary to consider using other more suitable Blocking Buffer according to the specific situation. 3. Please keep the Western blotting membrane or immunohistochemical slice moist, otherwise it may produce abnormal background. 4. This product couldn’t be used for clinical diagnosis or treatment, food or medicine, and can’t be stored in residence. 5. For your safety and health, please wear the lab coat and disposable gloves before the experiments. |
Other Clones
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Other Formats
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Biotin
Chromogenic Biotin
Cyanine 3
Cyanine 5
Cyanine 5.5
Cyanine 7
Elab Fluor®405
Elab Fluor®430
Elab Fluor®488
Elab Fluor®568
Elab Fluor®594
Elab Fluor®647
Elab Fluor®680
Elab Fluor®700
Elab Fluor®750
Elab Fluor®Red 780
Elab Fluor®Violet 450
FITC
HRP
NHS-Biotin
NHS-LC-LC-Biotin
Sulfo-NHS-Biotin
Sulfo-NHS-LC-LC-Biotin
Unconjugated
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