5× SDS Loading Buffer (E-BC-R288)
For research use only.
| Product Introduction |
This product is a protein loading buffer for 5× SDS Loading Buffer electrophoresis. The main components include SDS, bromophenol blue, reducing agent, etc. Mixed in proportion with proteins at heating can make proteins carry a large number of negative charges and eliminate their own charge differences; at the same time, the hydrogen bonds and disulfide bonds within and between molecules of proteins are disconnected, and the secondary and tertiary structures are destroyed, thereby controlling the migration rate of proteins only related to their molecular weight. This product adopts improved reductant to replace DTT with irritating odor and toxicity, which is mild and safe with better performance. |
| Applications | WB |
| Storage | Store at -20°C for 12 months; Store at 2-8°C for 6 months. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| Cautions |
1.When this product is stored at -20℃, precipitation may occur and must be completely melted before use. If insoluble substances such as precipitate are found, they can be heated in a 50℃ water bath for about 5-15 minutes until completely melted. Store the product in separate containers according to usage and not to perform repeated freeze-thaw treatment. 2.If the temperature is too high (e.g., more than 100℃) or too long (e.g., more than 15 min) when the mixed sample is heated in a water bath, it may cause protein degradation or abnormal color of the indicator in the loading buffer. 3.When using this product, it may become viscous, adhere to the walls, and not easily adsorb, which are all normal phenomena. 4.This product is suitable for denatured polyacrylamide gel electrophoresis. 5.This product is for professional scientific research only. 6.For your safety and health, please wear a lab coat and disposable gloves. |
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Biotin
Chromogenic Biotin
Cyanine 3
Cyanine 5
Cyanine 5.5
Cyanine 7
Elab Fluor®405
Elab Fluor®430
Elab Fluor®488
Elab Fluor®568
Elab Fluor®594
Elab Fluor®647
Elab Fluor®680
Elab Fluor®700
Elab Fluor®750
Elab Fluor®Red 780
Elab Fluor®Violet 450
FITC
HRP
NHS-Biotin
NHS-LC-LC-Biotin
Sulfo-NHS-Biotin
Sulfo-NHS-LC-LC-Biotin
Unconjugated
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