6-Phosphgluconic Acid (6-PGA) Fluorometric Assay Kit (E-BC-F152)
For research use only.
| Detection Principle |
6-phosphgluconic acid (6-PGA) is an intermediate in the pentose phosphate pathway. In the pentose phosphate pathway, 6-PGA is converted by 6-phosphogluconate dehydrogenase to ribulose-5-phosphate, which is a precursor for nucleic acid synthesis. The detection principle of this kit: 6-PGA is converted under enzymatic catalysis, and the fluorescent substrate is transformed into a fluorescent substance. The content of 6-PGA in the sample can be determined by measuring the fluorescence signal of fluorescent substance at an excitation wavelength of 535 nm and an emission wavelength of 587 nm. |
| Sample Type | Animal tissue,Cell |
| Detection Method | Fluorometric method |
| Detection Instrument | Fluorescence microplate reader (Ex/Em=535 nm/587 nm) |
| Research Area | Glycolysis And Lipid Metabolism |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 6.27 μmol/L |
| Detection Range | 6.27-100 μmol/L |
| Precision | inter-assay CV: 5.5%-8.6% | intra-assay CV: 2.1%-3.5% |
| Sample Volume | 20 μL(tissue homogenate/cell homogenate) |
| Assay Time | 1 h |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse liver tissue homogenate | 1 |
| 10% Mouse kidney tissue homogenate | 1 |
| 10% Mouse spleen tissue homogenate | 1 |
| 10% Mouse lung tissue homogenate | 1 |
| 10% Mouse heart tissue homogenate | 1 |
| 10% Porcine liver tissue homogenate | 1 |
| 1×10^6 293T Cells | 1 |
| 1×10^6 HL-60 Cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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