A-CoA(Acetyl Coenzyme A) ELISA Kit (E-EL-0125)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Universal A-CoA. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Universal A-CoA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Universal A-CoA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Universal A-CoA. You can calculate the concentration of Universal A-CoA in the samples by comparing the OD of the samples to the standard curve.

Acetyl-CoA is a component of cellular respiration that is mainly used to add acetyl groups in biochemical reactions. These reactions involve the metabolism of proteins, carbohydrates, and lipids, providing energy sources such as adenosine triphosphate (ATP), lactate, and ketones. Acetyl CoA, as a central metabolic intermediate, its abundance reflects the general energy state of cells, and its concentraion affects the activity or specificity of various enzymes, whether through conformational changes or changes in substrate availability. Acetyl CoA is the sole donor of protein acetylation and a key substrate in the tricarboxylic acid cycle, which is crucial for the biosynthesis of fatty acids and isoprene. In addition, acetyl CoA is synthesized within mitochondria, involving aerobic oxidation of glucose pyruvate, catabolism of certain amino acids, and β- Hydroxybutyrate Acetoacetate Acetyl CoA β- Oxidation. The sources of acetyl CoA include aerobic oxidation of sugars (glucose pyruvate) and fatty acids β- Oxidation, catabolism of certain amino acids, and oxidative breakdown of ketones. This indicates that acetyl CoA not only plays a core role in energy conversion, but also plays a crucial role in various metabolic pathways of cells.