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200μL $ 410.00
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For research use only.

Verified Samples Verified Samples in WB: Hela, Mouse kidney, Mouse lung, Rat kidney
Dilution WB 1:500-1:2000
Isotype IgG
Host Rabbit
Reactivity Human,  Mouse,  Rat
Applications WB
Clonality Polyclonal
Immunogen KLH conjugated Synthetic peptide corresponding to Mouse ACE2
Abbre ACE2
Synonyms ACE 2,  ACE related carboxypeptidase,  ACE-related carboxypeptidase,  ACE2,  ACEH,  Angi,  Angiotensin I Converting Enzyme (peptidyl dipeptidase A) 2,  Angiotensin converting enzyme 2,  Angiotensin converting enzyme homolog,  Angiotensin converting enzyme like protein
Swissprot
Calculated MW 92 kDa
Observed MW 95 kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Secreted and Cell membrane.
Concentration 330 μg/mL
Buffer PBS with 0.02% sodium azide, 1% protective protein and 50% glycerol, pH7.4
Purification Method Affinity purification
Research Areas Cancer,  Cell Biology,  Cardiovascular
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.
background The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63.
Cat.No. Product Name Sizes
E-AB-1003 Goat Anti-Rabbit IgG(H+L)(peroxidase/HRP conjugated) 500μL , 120μL , 60μL
E-AB-1043 Streptavidin(peroxidase/HRP conjugated) 500μL , 120μL , 60μL
E-AB-1194 HRP-Goat Anti-Rabbit IgG(H+L) preadsorbed 500μL , 120μL , 1mL
E-IR-R304A Western Blot Detection Kit 50Assays
E-IR-R304B High Accuracy and Absorbability Western Blot Detection Kit 50Assays
Other Clones

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Unconjugated

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