Acetate Kinase (AcK) Activity Colorimetric Assay Kit (E-BC-K910-M)
For research use only.
| Detection Principle |
Acetate kinase (AcK) is a member of the ASKHA (acetate and sugar kinases, Hsp70, actin) phosphotransferase superfamily and serves as a central enzyme in carbon and energy metabolism in prokaryotes. AcK catalyzes the acetate reaction with concomitant consumption of NADH. NADH exhibits a maximum absorption peak at 340 nm, and AcK activity is calculated by measuring the rate of decrease in absorbance at 340 nm. |
| Sample Type | Serum,Plasma,Animal tissue,Plant tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (340 nm) |
| Research Area | Lipid Metabolism , Metabolic Enzyme Activity |
| Other Reagents Required | PBS(0.01 M，pH 7.4) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.50 U/L |
| Detection Range | 0.50-125.00 U/L |
| Precision | inter-assay CV: 5.0%-7.2% | intra-assay CV: 2.2%-3.6% |
| Sample Volume | 20 μL |
| Assay Time | 50 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse liver tissue homogenization | 2-3 |
| 10% Mouse kidney tissue homogenization | 2-3 |
| 10% Mouse spleen tissue homogenization | 1 |
| 10% Mouse lung tissue homogenization | 1 |
| 10% Pig liver tissue homogenization | 1 |
| 1×10^6 K562 cells | 1 |
| 1×10^6 Molt-4 cells | 1 |
| 1×10^6 H9c2 cells | 1 |
| 1×10^6 A549 cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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