AF/LE Purified Anti-Mouse TNF-α Antibody[TN3-19.12] (AN008310)
For research use only.
| Alternate Names | Tumor necrosis factor ligand superfamily member, TNFSF, DIF, Tnfa, TNF-a, TNFSF2, Tnlg1f, Tnfsf1a, TNFalpha, TNF-alpha, ICD1, ICD2, Membrane Form, N-terminal fragment, NTF, Tnf, Cachectin, Tumor necrosis factor, Tumor necrosis factor ligand superfamily member 2, Tnfa, TNF-a, TNFalpha, TNF-alpha, Tnfsf1a, TNFSF2, Tnlg1f, TNFSF2, TNF, TNFa |
| Clone No | |
| Verified Samples | Verified Samples in FCM: HEK293T cells transfected with pcDNA3.1 plasmid encoding Mouse TNF-α gene |
| Dilution | FCM 2 μg/mL(0.5×106-1×106 cells) |
| Background | Tumor necrosis factor alpha (TNF-alpha, TNF- alpha, TNFA ), also known as Cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism. TNF-alpha is produced by several lymphoid cells as well as by astrocytes, endothelial cells, and smooth muscle cells. Mouse TNF-alpha consists of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 179 aa extracellular domain (ECD). |
| Immunogen | Recombinant Mouse TNF-α protein |
| Abbre | TNF-α |
| Swissprot | |
| Host | Armenian Hamster |
| Reactivity | Mouse |
| Clonality | Monoclonal |
| Purification Method | >98%, Protein A/G purified |
| Isotype | Armenian Hamster IgG2, κ |
| Applications |
FCM
|
| Cellular Localization |
Cell membrane, Membrane, Secreted
|
| Concentration |
≥ 1 mg/mL
|
| Conjugation | None (AF/LE) |
| Storage Buffer | Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method. |
| Storage | Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions. |
| Expiration Date | 12 months |
| Shipping | Ice bag |
Other Clones
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Other Formats
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Elab Fluor®647
None (AF/LE)
PE/Cyanine 7
Unconjugated
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