Albumin (ALB) Colorimetric Assay Kit (Bromocresol Green Method) (E-BC-K057-M)
For research use only.
| Detection Principle | Bromocresol green (BCG) is widely used as protein staining agent. BCG can combine the albumin in pH 4.0-4.2 to form an albumin-BCG complex. And the color changed from yellow to green. The depth of color is proportional to the concentration of albumin. The content of albumin in serum can be calculated indirectly by measuring the OD value at 630 nm. |
| Synonyms | ALB |
| Sample Type | Serum,Plasma |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (620-640 nm) |
| Research Area | Liver And Renal Function , Amino Acids And Proteins |
| Other Reagents Required | Normal saline (0.9% NaCl) |
| Storage | This product can be stored at 2-8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components. |
| Valid Period | 12 months |
| Sensitivity | 0.08 g/L |
| Detection Range | 0.08-15 g/L |
| Precision | inter-assay CV: 4.6% | intra-assay CV: 1.5% |
| Assay Time | 20 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 8-15 |
| Human plasma | 8-15 |
| HepG2 supernatant | 1 |
| Mouse plasma | 8-15 |
| Rat serum | 8-15 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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