Alkaline Phosphatase (ALP) Activity Colorimetric Assay Kit(Method of NPP-AMP) (E-BC-K1304-M)
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For research use only.
| Detection Principle | Using 4-Nitrophenyl phosphate (4-NPP) as the substrate and 2-Amino-2-methyl-1-propanol (AMP) as the receptor substance for the phosphate group, alkaline phosphatase (ALP) catalyzes the reaction to generate free 4-nitrophenol (4-NP). The latter converts into a quinone structure in an alkaline solution and exhibits a darker yellow color. The activity of ALP in the sample can be calculated based on the rate of increase in absorbance at 405 nm. |
| Sample Type | Animal tissue,Cell,Serum,Plasma |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (400-410 nm, optimum wavelength: 405 nm) |
| Research Area | Liver And Renal Function |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 6.09 U/L |
| Detection Range | 6.09-4500 U/L |
| Precision | inter-assay CV: 2.6%-4.1% | intra-assay CV: 1.3%-3.1% |
| Sample Volume | 5 μL |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Rats serum | 1 |
| Horse serum | 1 |
| Human plasma | 1 |
| Rabbit serum | 1 |
| Porcine serum | 1 |
| 10% Mouse liver tissue homogenate | 1 |
| 10% Mouse kidney tissue homogenate | 1 |
| 2×10^6 Hela cells | 1 |
| 2×10^6 HepG2 cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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