γ-Aminobutyric Acid (GABA) Colorimetric Assay Kit (E-BC-K852-M)
For research use only.
| Detection Principle | Phenol and sodium hypochlorite react with GABA to produce a blue-green product, which has maximum absorbance at 640 nm. GABA content can be calculated with the absorbance at 640 nm. |
| Sample Type | Plant,Animal tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (630-650 nm, optimum wavelength: 640 nm) |
| Research Area | Amino Acids And Proteins , Plant Physiology Series |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.06 μmol/mL |
| Detection Range | 0.06-10.0 μmol/mL |
| Precision | inter-assay CV: 6.8% | intra-assay CV: 0.04 |
| Sample Volume | 30 μL(tissue homogenate) |
| Assay Time | 40 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Epipremnum aureum tissue homogenization | 1 |
| 10% Green pepper tissue homogenization | 1 |
| 10% Chinese yam tissue homogenization | 1 |
| 10% Rat heart tissue homogenization | 1 |
| 10% Rat liver tissue homogenization | 1 |
| 10% Rat kidney tissue homogenization | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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