Ammonia Colorimetric Assay Kit (E-BC-K777-M)
For research use only.
| Detection Principle |
Ammonia is an important source of nitrogen for living systems and is necessary for the synthesis of amino acids. Ammonia is a metabolite produced by the deamination of amino acids. It plays an important role in both normal and abnormal animal physiology. In severe liver diseases, ammonia can not be removed from the circulation, resulting in increased blood ammonia, which can lead to hepatic encephalopathy (hepatic coma). In clinical practice, blood ammonia measurement is an important experimental diagnosis and monitoring index of hepatic encephalopathy. The detection principle of this kit: enzyme-catalyzed ammonia reaction consumes NADH, causing its absorbance to decline at 340 nm. By measuring the rate of absorbance decline at 340 nm, the ammonia content can be measured. |
| Sample Type | Serum,Plasma,Urine,Animal and plant tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (330-350 nm, optimum wavelength: 340 nm) |
| Research Area | Amino Acids And Proteins |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.01 mmol/L |
| Detection Range | 0.01-2.00 mmol/L |
| Precision | inter-assay CV: 7.2-9.6% | intra-assay CV: 0.9-3.8% |
| Sample Volume | 40 μL |
| Assay Time | 1 h 10 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Mouse serum (plasma) | 1 |
| Rat serum | 1 |
| Human serum | 1 |
| Human urine | 5-10 |
| 10% Mouse liver tissue homogenate | 1 |
| 10% Mouse kidney tissue homogenate | 1 |
| 10% Mouse brain tissue homogenate | 1 |
| 10% Broccoli tissue homogenate | 1 |
| 10% Bean sprout tissue homogenate | 1 |
| 1×10^6 Molt-4 cells | 1 |
| 1×10^6 Jurkat cells | 1 |
| 1×10^6 293T cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
Other Clones
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Biotin
Chromogenic Biotin
Cyanine 3
Cyanine 5
Cyanine 5.5
Cyanine 7
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Elab Fluor®430
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FITC
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Unconjugated
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