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100μL $ 260.00
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For research use only.

Verified Samples Verified Samples in WB: Mouse pancreas
Dilution WB 1:500-1:1000
Isotype Rabbit IgG
Host Rabbit
Reactivity Mouse
Applications WB
Clonality Polyclonal
Immunogen Recombinant Human AMY1A protein expressed by Mammalian
Abbre AMY1A
Synonyms 1,  4-alpha-D-glucan glucanohydrolase 1,  AMY1,  AMY1A,  Alpha-amylase 1A,  EC:3.2.1.1 ,  Salivary alpha-amylase
Swissprot
Calculated MW 58 kDa
Observed MW 58 kDa

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Concentration 1 mg/mL
Buffer PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4
Purification Method Antigen Affinity Purification
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.
background Calcium-binding enzyme that initiates starch digestion in the oral cavity.Catalyzes the hydrolysis of internal (1->4)-alpha-D-glucosidic bonds,yielding a mixture of maltose,isomaltose,small amounts of glucose as well as small linear and branched oligosaccharides called dextrins.
Cat.No. Product Name Sizes
E-AB-1003 Goat Anti-Rabbit IgG(H+L)(peroxidase/HRP conjugated) 500μL , 120μL , 60μL
E-AB-1043 Streptavidin(peroxidase/HRP conjugated) 500μL , 120μL , 60μL
E-AB-1194 HRP-Goat Anti-Rabbit IgG(H+L) preadsorbed 500μL , 120μL , 1mL
E-IR-R304A Western Blot Detection Kit 50Assays
E-IR-R304B High Accuracy and Absorbability Western Blot Detection Kit 50Assays
Other Clones

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Unconjugated

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