Anti-His(HHHHHH) Affinity Agarose (EA-IP-008)
For research use only.
| Parameter Information | 1.Application Scope Affinity purification and immuno(co)precipitation of His-tagged fusion secreted proteins. His tag can be located at the N-terminus, C-terminus, or middle of the protein, such as the N-terminus His fusion protein (His-Protein), C-terminus His fusion protein (Protein-His), and Met modified intermediate His fusion protein (Met-His-Protein). Can only be used for affinity purification of secreted proteins. 2.Antibody Attributes His-Tag Mouse mAb：Mouse IgG. 3.Binding substrate Agarose gel particles, average particle size 100 μ M. 4.Loading Capacity 1 mL of Sepharose 4B agarose particles, covalently conjugated to 6 mg of mouse-derived IgG. A 1 mL affinity gel can purify or precipitate at least 1.2 mg of His fusion protein. 5.Liquid storage composition PBS containing preservatives and 50% glycerol. |
| Applications | IP/COIP |
| Storage | -20℃, 12 months |
| Shipping | ice bag |
| Notes |
1. This product is limited to scientific research by professionals and cannot be used for clinical diagnosis or treatment. 2. For your safety and health, please wear laboratory clothes and disposable gloves for operation. 3. This product is in the form of gel suspension, and the content of affinity gel is 50%. Before use, gently re-suspend the gel suspension, and then use it as required. 4. If the gel fades slightly, it is normal and will not affect the experimental results. 5. It is best to prepare and use the IP-WB sample on site to avoid affecting the experimental results. 6. When a little gel is mixed with the protein loading buffer, it is normal for the WB glue to appear light pink, which does not affect the experimental results. 7. Do not dry the gel, do not sonicate the gel, and do not allow the acid treatment of gel to exceed 10 minutes. 8. The amount of gel mentioned in the method is the demonstration amount prepared in small quantities, and the specific amount should be adjusted according to the actual situation. |
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