Ascorbate Peroxidase (APX) Activity Assay Kit (E-BC-K353-M)
For research use only.
| Detection Principle | Ascorbate Peroxidase (APX) can catalyze the reaction between ascorbic acid (ASA) and hydrogen peroxide (H2O2), and ASA can be oxidized to monodehydroascorbic acid (MDASA). The absorbance of solution at 290 nm will decline as the oxidation of ASA. The APX activity can be calculated by detecting the decrease of A290. |
| Sample Type | Plant tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (280-300 nm) |
| Other Reagents Required | Double distilled water, Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.011 U/L |
| Detection Range | 0.011-1.47 U/L |
| Precision | inter-assay CV: 2.6%-9.3% | intra-assay CV: 5.7%-6.9% |
| Sample Volume | 20 μL |
| Assay Time | 20 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Epipremnum aureum tissue homogenate | 1 |
| 10% Carrot tissue homogenate | 1 |
| 10% Green pepper tissue homogenate | 1 |
| 10% Mushrooms tissue homogenate | 1 |
| 10% Avocado tissue homogenate | 1 |
| 10% Pumpkin tissue homogenate | 1 |
| 10% Chive tissue homogenate | 1 |
| 10% Spinach tissue homogenate | 1 |
| 10% Bok choy tissue homogenate | 1 |
| 10% Oyster mushroom tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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