Aspartate (Asp) Colorimetric Assay Kit (E-BC-K849-M)
For research use only.
| Detection Principle | L-aspartate (L-Aspartate) is an α-amino acid, which is the same acidic amino acid as glutamate. It is one of the non-essential amino acids in the human body. The detection principle of this kit is that the enzyme catalyzes aspartic acid and substrate to produce glutamic acid, and glutamic acid is further catalyzed by the enzyme to produce a product, which will be reduced to orange yellow product under the action of electronic coupler, and the maximum absorption peak is detected at 450 nm. |
| Sample Type | Serum,Plasma,Animal tissue,Plant tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (440-465 nm, optimum wavelength: 450 nm) |
| Research Area | Amino Acids And Proteins |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 1.7 μmol/L |
| Detection Range | 1.7-471.7 μmol/L |
| Precision | inter-assay CV: 4.4-9.0% | intra-assay CV: 1.9-4.3% |
| Sample Volume | 40 μL |
| Assay Time | 1 h 20 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse liver tissue homogenate | 3-4 |
| 10% Mouse heart tissue homogenate | 3-4 |
| 10% Rat liver tissue homogenate | 3-4 |
| 10% Rat kidney tissue homogenate | 3-4 |
| 10% Rat heart tissue homogenate | 3-4 |
| Dog serum | 1 |
| Horse plasma | 1 |
| 1.0 × 10^6 CHO cells | 1 |
| 1.0 × 10^6 Jurkat cells | 1 |
| 1.0 × 10^6 Molt-4 cells | 1 |
| 1.0 × 10^6 293T cells | 1 |
| 1.0 × 10^6 HL-60 cells | 1 |
| 1.0 × 10^6 THP-1 cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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