ATP Colorimetric Assay Kit (E-BC-K157-M)
For research use only.
| Detection Principle | Creatine kinase catalyzes adenosine triphosphate and creatine to produce creatine phosphate. The content of phosphocreatine was determined by colorimetric method to reflect the content of ATP. |
| Synonyms | ATP |
| Sample Type | Animal tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (630-640 nm, optimum wavelength: 636 nm) |
| Research Area | Oxidative Stress , Disulfidptosis , Energy Metabolism |
| Storage | This product can be stored at 2-8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components. |
| Valid Period | 12 months |
| Sensitivity | 0.01 mmol/L |
| Detection Range | 0.03-1.5 mmol/L |
| Precision | inter-assay CV: 5.4% | intra-assay CV: 5.1% |
| Sample Volume | 60 μL(tissue homogenate) |
| Assay Time | 1 h |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Rat muscle tissue homogenate | 2-4 |
| 10% Rat liver tissue homogenate | 2-4 |
| 10% Mouse brain tissue homogenate | 2-4 |
| 10% Rat kidney tissue homogenate | 2-4 |
| 10% Rat lung tissue homogenate | 2-4 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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