ATP Colorimetric Assay Kit (Enzyme Method) (E-BC-K774-M)
For research use only.
| Detection Principle | Adenosine Triphosphate (ATP) is a high-energy phosphate compound, which is the most direct energy source in the body, and its content is directly related to the state of energy metabolism in the body. Hexokinase (HK) catalyzes the generation of glucose and ATP into glucose-6-phosphate, and glucose-6-phosphate dehydrogenase (G6PD) further catalyzes the dehydrogenation of glucose-6-phosphate into NADPH. NADPH can react with the color developer, and the product has a characteristic absorption peak at 460 nm to determine the ATP content. |
| Sample Type | Animal tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (450-470 nm, optimum wavelength: 460 nm) |
| Research Area | Tricarboxylic Acid（TCA）Cycle , Glycolysis And Lipid Metabolism , Energy Metabolism |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.72 μmol/L |
| Detection Range | 0.72-40.00 μmol/L |
| Precision | inter-assay CV: 1.7-6.4% | intra-assay CV: 1.5-2.2% |
| Sample Volume | 200 μL(tissue homogenate) |
| Assay Time | 40 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse heart tissue homogenate | 1 |
| 10% Mouse kidney tissue homogenate | 1 |
| 10% Mouse muscle tissue homogenate | 1 |
| 10% Mouse liver tissue homogenate | 1 |
| 10% Mouse brain tissue homogenate | 1 |
| 10% Fish muscle tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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