ATPase/GTPase Activity Colorimetric Assay kit (E-BC-K826-M)
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For research use only.
| Detection Principle |
Adenosine triphosphatase (ATPase) is a class of enzymes that catalyze the conversion of ATP into ADP and inorganic phosphate. The catalytic process releases energy and drives a variety of cellular activities, such as ion transmembrane transport and muscle contraction. Guanosine triphosphatase (GTPase) catalyzes the conversion of GTP into GDP and inorganic phosphate. GTPases play key roles in signal transduction, protein synthesis, and cytoskeleton regulation. |
| Sample Type | Serum,Plasma,Animal tissue,Plant tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (600-640 nm) |
| Other Reagents Required | Normal saline (0.9% NaCl),double distilled water |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.03 U/L |
| Detection Range | 0.03-5.00 U/L |
| Precision | inter-assay CV: 6.7%-10.6% | intra-assay CV: 1.7%-6.8% |
| Sample Volume | 100 μL |
| Assay Time | 1 h 10 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse liver tissue homogenization | 15-30 |
| 10% Mouse kidney tissue homogenization | 24-50 |
| 10% Epipremnum aureum leaf tissue homogenization | 6-15 |
| 10% Schefflera octophylla leaf tissue homogenization | 6-15 |
| Mouse serum | 8-15 |
| Human plasma | 8-15 |
| 1×10^6 293T cells | 2-8 |
| 1×10^6 A549 cells | 2-8 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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