BCA Protein Colorimetric Assay Kit (E-BC-K318-M)
For research use only.
| Detection Principle | Cu2+ can be reduced to Cu+ by protein in alkaline condition. Cu+ can combine with BCA reagent and form purple complex, which has a maximum absorption peak at 562 nm. The absorbance value is proportional to the protein concentration. Therefore, the protein concentration can be calculated according to the OD value. |
| Synonyms | TP |
| Sample Type | Serum,Plasma,Cell culture supernatant,Tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (540-590 nm, optimum wavelength: 562 nm) |
| Research Area | Amino Acids And Proteins |
| Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at room temperature for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.0165 mg/mL |
| Detection Range | 0.0165-1 mg/mL |
| Precision | inter-assay CV: 4.5% | intra-assay CV: 2.2% |
| Assay Time | 40 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse brain tissue homogenization | 8-12 |
| 10% Mouse kidney tissue homogenization | 8-12 |
| Human serum | 100-200 |
| 10% Rat liver tissue homogenization | 15-20 |
| 10% Mouse heart tissue homogenization | 8-12 |
| Rat serum | 100-200 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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