Biotin Colorimetric Assay Kit (E-BC-K1105-M)
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For research use only.
| Detection Principle |
Biotin is an important biological molecule that is widely used to label antibodies or other proteins in biotinylated reactions. The detection principle: Biotin can compete with avidin for binding to the dye, causing a decrease in the absorbance value at 500 nm, calculate the content of biotin. |
| Sample Type | Antibodies,Proteins |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (500 nm) |
| Research Area | Glycolysis And Lipid Metabolism |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 12.60 μmol/L |
| Detection Range | 12.60-300.00 μmol/L |
| Precision | inter-assay CV: 3.5-6.0% | intra-assay CV: 1.2-3.4% |
| Sample Volume | 20 μL |
| Assay Time | 40 min |
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