BSG Polyclonal Antibody (E-AB-40298)
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For research use only.
| Verified Samples |
Verified Samples in WB: Mouse heart, Rat liver, Rat heart, Rat kidney, Rat brain Verified Samples in IHC: Rat liver, Rat stomach |
| Dilution | WB 1:1000-1:2000, IHC 1:100-1:300 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Mouse, Rat |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Immunogen | Recombinant Rat Basigin protein |
| Abbre | BSG |
| Synonyms | 5A11 antigen, 5F7, BASI, Basigin, Basigin (Ok blood group), Blood brain barrier HT7 antigen, Bsg, CD 147, CD147, CD147 antigen, Collagenase stimulatory factor, EMMPRIN, Extracellular matrix metalloproteinase inducer, Leukocyte activation antigen M6, M 6, M6, M6 leukocyte |
| Swissprot | |
| Calculated MW | 30 kDa,40-60 kDa |
| Observed MW |
30 kDa,40-60 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cell membrane |
| Concentration | 1 mg/mL |
| Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Antigen Affinity Purification |
| Research Areas | Cancer, Cardiovascular, Metabolism, Immunology, Neuroscience |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | BSG,also named as 5F7,Basigin,EMMPRIN,TCSF and CD147,plays pivotal roles in spermatogenesis,embryo implantation,neural network formation and tumor progression. It stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS).CD147 may target monocarboxylate transporters SLC16A1,SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. It seems to be a receptor for oligomannosidic glycans. CD147 is a receptor of CypA,inducing matrix metalloproteinase expression and mediating the degradation of the extracellular matrix,plays an important role in tumorigenesis and invasion in oral cancer. It has been considered as an objective and effective marker to predict invasion and prognosis in various cancers. |
Other Clones
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Other Formats
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Unconjugated
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