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For research use only.

Detection Principle Elabscience® Calcein AM/PI Double Staining Kit can be used to distinguish dead cells and living cells in mammals with esterase activity. Calcein AM is the addition of acetyl methoxy methyl ester (AM) group to traditional Calcein, which increases hydrophobicity and can easily penetrate the living cell membrane and enter the cell. Calcein AM itself has no fluorescence. After entering the cell, it is hydrolyzed by endogenous esterase in the cell to produce Calcein, a polar molecule with strong negative charge and cannot be retained in the cell through the cell membrane, while Calcein can emit strong green fluorescence ( Ex / Em = 494nm / 517nm ). Due to the lack of esterase, dead cells cannot or rarely produce Calcein, so only living cells are stained with strong green fluorescence, and dead cells cannot be stained or stained very weakly. The selective membrane permeability of dead cells is lost, and Propidium Iodide ( PI ) can enter the cell to specifically bind to double-stranded DNA and produce strong red fluorescence(Ex/Em = 535nm/617nm) to label dead cells. Therefore, the combination of Calcein AM and PI can perform double fluorescence staining on living cells and dead cells at the same time, which can be used for the detection of cell activity and cytotoxicity.
Detection Method Fluorometric method, Flow cytometry
Sample Type Cell samples
Assay Time 30 min
Detection Instrument Flow Cytometer;Fluorescence Microscope
Dye Type Calcein/PI
Ex/Em 494/517,535/617
Channel Set FITC, PE/PerCP/Cy5.5
Filter Set FITC, TRITC
Other Reagents Required PBS (pH7.2-7.6)(E-BC-R187).
Storage This product can be stored at -20°C for 12 months with shading light.
Expiration date 12 months
Shipping Ice bag
Cat.No. Product Name Sizes
E-CK-A153 Calcein AM Assay Buffer 100mL
E-CK-A164 Calcein AM Solution (100 μM) 500Tests , 100Tests , 500Tests×10
E-CK-A165 Propidium Iodide (PI) Solution(750 μM) 500Tests , 100Tests , 500Tests×10
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