For research use only.
| Verified Samples |
Verified Samples in WB: Hela, A375 |
| Dilution | WB 1:500-1:2000 |
| Clonality | Polyclonal |
| Immunogen | Fusion protein of human CANT1 |
| Abbre | CANT1 |
| Synonyms | Apyrase, Apyrase homolog, CANT 1, CANT1, Ca2+ dependent endoplasmic reticulum nucleoside diphosphatase, Calcium activated nucleotidase 1, Putative MAPK activating protein PM09, Putative MAPK-activating protein PM09, Putative NF kappa B activating protein 10 |
| Swissprot | |
| Calculated MW | 45 kDa |
| Observed MW |
Refer to figures
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Endoplasmic reticulum membrane. Golgi apparatus>Golgi stack membrane. Processed form: Secreted. |
| Concentration | 0.7 mg/mL |
| Buffer | PBS with 0.05% NaN3 and 40% Glycerol,pH7.4 |
| Purification Method | Antigen affinity purification |
| Research Areas | Epigenetics and Nuclear Signaling, Signal transduction |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | This protein encoded by this gene belongs to the apyrase family. It functions as a calcium-dependent nucleotidase with a preference for UDP. Mutations in this gene are associated with Desbuquois dysplasia with hand anomalies. Alternatively spliced transcript variants have been noted for this gene. |
Other Clones
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