Caspase 3/7 and Annexin V Double Staining Apoptosis Kit (E-CK-A831)
For research use only.
| Detection Principle | The Caspase 3/7 Substrates（Green）are based on novel fluorogenic DNA dyes that have been coupled to the caspase 3/7 recognition sequence (DEVD),which is both non-fluorescent and nonfunctional as a DNA dye.When it rapidly crosses cell membranes to enter the cytoplasm and cleaved by caspase 3/7 to form a high-affinity DNA dye that stains the nucleus bright green. Thus, the Caspase 3/7 substrates allow detection of caspase 3/7 activity and visualization of apoptotic nuclear morphology simultaneously. Annexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. Annexin V-APC, the APC-conjugated format, binds specifically to the PS on the outer leaflet apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscopy. Elabscience® Caspase 3/7 and Annexin V Double Staining Apoptosis Kit includes Caspase 3/7 Substrates(Green)和Annexin V-APC Reagent, enables the user to detect two important apoptosis events, caspase 3/7 activation and phosphatidylserine (PS) translocation. |
| Detection Method | Fluorometric method, Flow cytometry |
| Sample Type | Cell samples |
| Assay Time | 0.5 hour |
| Detection Instrument | Flow Cytometer |
| Dye Type | FITC;APC |
| Ex/Em | Caspase 3: Ex/Em=490nm/535nm; Annexin V:Ex/Em=650nm/660nm |
| Channel Set | FITC;APC |
| Other Reagents Required | PBS, cell culture medium, fetal bovine serum |
| Storage | This product can be stored at 2~8℃ for 1 year in the dark. |
| Expiration date | 12 months |
| Shipping | Ice bag |
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