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Caspase 9 Activity Assay Kit(Colorimetric Method) (E-CK-A389)

All Size Price Qty
100Assays $ 228.00
50Assays $ 136.00
20Assays $ 68.00
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For research use only.

Detection Principle Caspase 9, also known as ICE-LAP6 or Mch6, is a relatively upstream caspase in the process of apoptosis signal transduction. After mitochondria release cytochrome c, caspase 9 can form complex with cytochrome c and Apaf1 and can be activated. Activated caspase 9 can activate caspase 3, the most critical enzyme of apoptosis, thereby promoting subsequent apoptotic signals. The activation of caspase 9 can be regulated by phosphorylation. This caspase 9 activity assay kit is based on caspase 9 that can catalyze the substrate Ac-LEHD-pNA to generate yellow pNA (p-Nitroaniline). The pNA has a strong absorption near 405 nm, The activity of caspase 9 can be calculated by determining the absorbance at 405 nm.
Detection Method Colorimetric method
Sample Type Cell and tissue samples
Assay Time 4-6 hours
Detection Instrument Spectrophotometer
Other Reagents Required PBS(E-BC-R187), Bradford Protein Determination Kit(E-BC-K168-S or E-BC-K168-M)
Storage This product can be stored at -20°C for 12 months with shading light.
Expiration date 6 months
Shipping Ice bag
Cat.No. Product Name Sizes
E-CK-A382 Caspase 2 Activity Assay Kit(Colorimetric Method) 100Assays , 50Assays , 20Assays
E-CK-A383 Caspase 3/7 Activity Assay Kit(Colorimetric Method) 100Assays , 50Assays , 20Assays
E-CK-A384 Caspase 4 Activity Assay Kit(Colorimetric Method) 100Assays , 50Assays , 20Assays
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