Caspase 9 Activity Assay Kit(Colorimetric Method) (E-CK-A389)
For research use only.
| Detection Principle | Caspase 9, also known as ICE-LAP6 or Mch6, is a relatively upstream caspase in the process of apoptosis signal transduction. After mitochondria release cytochrome c, caspase 9 can form complex with cytochrome c and Apaf1 and can be activated. Activated caspase 9 can activate caspase 3, the most critical enzyme of apoptosis, thereby promoting subsequent apoptotic signals. The activation of caspase 9 can be regulated by phosphorylation. This caspase 9 activity assay kit is based on caspase 9 that can catalyze the substrate Ac-LEHD-pNA to generate yellow pNA (p-Nitroaniline). The pNA has a strong absorption near 405 nm, The activity of caspase 9 can be calculated by determining the absorbance at 405 nm. |
| Detection Method | Colorimetric method |
| Sample Type | Cell and tissue samples |
| Assay Time | 4-6 hours |
| Detection Instrument | Spectrophotometer |
| Other Reagents Required | PBS(E-BC-R187), Bradford Protein Determination Kit(E-BC-K168-S or E-BC-K168-M) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Expiration date | 6 months |
| Shipping | Ice bag |
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