Cathepsin B Activity Fluorometric Assay Kit (E-BC-F059)
For research use only.
| Detection Principle |
Cathepsin B is a cysteine proteolytic enzyme in lysosomes. It is responsible for the hydrolysis, degradation and processing of proteins in organisms, thus maintaining a precise balance between protein synthesis and degradation. The abnormal proteolytic activity of cathepsin and the alteration of its expression pattern are involved in the key steps of the tumor disease process and can be used as markers for certain cancer types, serving as effective regulatory factors for cancer occurrence. As an important type of biomarker, the research on cathepsin is crucial for the study of drug targets and drug development. The detection principle of this kit: Cathepsin B specifically cleaves the fluorescent substrate, and the fluorescent group of the product emits fluorescence. Fluorescence is detected at an excitation wavelength of 355 nm and an emission wavelength of 460 nm. |
| Sample Type | Animal tissue,Plant tissue,Cell |
| Detection Method | Fluorometric method |
| Detection Instrument | Fluorescence microplate reader (Ex/Em=355 nm/460 nm) |
| Research Area | Amino Acids And Proteins |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.009 U/L |
| Detection Range | 0.009-0.351 U/L |
| Precision | inter-assay CV: 5.1%-9.9% | intra-assay CV: 2.1%-4.9% |
| Sample Volume | 20 μL(tissue homogenate/cell homogenate) |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse liver tissue homogenate | 3-10 |
| 10% Mouse kidney tissue homogenate | 2-3 |
| 10% Mouse muscle tissue homogenate | 1 |
| 10% Mouse spleen tissue homogenate | 3-10 |
| 10% Mouse lung tissue homogenate | 1 |
| 10% Potato tissue homogenate | 1 |
| 10% Corn tissue homogenate | 1 |
| 1×10^6 HL-60 cells | 1 |
| 1×10^6 RAW 264.7 cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
Other Clones
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Biotin
Chromogenic Biotin
Cyanine 3
Cyanine 5
Cyanine 5.5
Cyanine 7
Elab Fluor® 430
Elab Fluor® 488
Elab Fluor® 568
Elab Fluor® 594
Elab Fluor® 647
Elab Fluor® 680
Elab Fluor® 700
Elab Fluor® 750
Elab Fluor® Red 780
Elab Fluor® Violet 450
Elab Fluor®488
Elab Fluor®594
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Elab Fluor®Violet 450
FITC
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NHS-Biotin
NHS-LC-LC-Biotin
Sulfo-NHS-Biotin
Sulfo-NHS-LC-LC-Biotin
Unconjugated
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