CCR2/cd192 Polyclonal Antibody (AN100002P)
For research use only.
| Verified Samples |
Verified Samples in WB: A431 Verified Samples in IP: Hela |
| Dilution | WB 1:500-1:2000, IP 4-6 μL/mg of lysate |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB, IP |
| Clonality | Polyclonal |
| Immunogen | A synthetic peptide corresponding to the N-terminus of the Human CCR2/cd192 |
| Abbre | CCR2 |
| Synonyms | CKR, CMKBR, CC-CKR, CCR, CCR2, CC-CKR-2, CCR-2, CCR2A, CCR2B, CD192, CKR2, CKR2A, CKR2B, CMKBR2, MCP-1-R, FLJ78302, C C chemokine receptor type 2, C C CKR 2, CC chemokine receptor type 2, C-C chemokine receptor type 2, CC CKR 2, C-C CKR-2, CCCKR2, CCR 2, CCR1L, CCR5L, CD192 antigen, Chemokine C C motif receptor 2, Chemokine CC Motif Receptor 2, CKR 2, cluster of differentiation 192, MCP 1 R, MCP1 RECEPTOR, MCP1R, MCP-1-R, Monocyte chemoattractant protein 1 receptor, Monocyte Chemotactic Protein 1 Receptor |
| Swissprot | |
| Calculated MW | 45 kDa |
| Observed MW |
45 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cell membrane |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A & Antigen Affinity |
| Research Areas | Immunology, Microbiology, Signal Transduction, Cancer, Cardiovascular |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | The C-C motif chemokine receptor-2 (CCR2) is a G protein-coupled receptor (GPCR), made up of a carboxy-terminus, extracellular amino terminus, and a hydrophobic transmembrane domain consisting of 7 amino acid segments that mediates agonist-dependent calcium mobilization and inhibition of adenylyl cyclase. CCR2 is expressed on monocytes, immature dendritic cells, and T-cell subpopulations, and mediates their migration towards endogenous CCL2. CCR2 is necessary for macrophage-dependent inflammatory responses and the development of atherosclerosis. In mice, CCR2 deficiency reduced macrophage content, increased adiponectin expression, ameliorated hepatic steatosis, and improved systemic glucose homeostasis and insulin sensitivity. Resistance to HIV-1 infection or delayed progression to AIDS may be linked to CCR2 polymorphisms. Furthermore, CCR2 mRNA was highly expressed in prostate cancer (PCa) metastatic tissues compared with benign prostate tissues according to real-time RT-PCR3, suggesting that CCR2 may contribute to PCa development. |
Other Clones
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Unconjugated
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