CD10 Monoclonal Antibody (AN200185P)
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For research use only.
| Verified Samples |
Verified Samples in WB:?Raji Verified Samples in IHC: Human lymphoma, Human Submandibular gland inflammation, Human uterus cancer Verified Samples in IP: Raji |
| Dilution | WB 1:500-1:2000, IHC-P 1:20-1:100, IP 1-5 μL/mg of lysate |
| Isotype | IgG1 |
| Host | Mouse |
| Reactivity | Human |
| Applications | WB, IHC-P, IP |
| Clonality | Monoclonal |
| Immunogen | Recombinant Human CD10 Protein |
| Abbre | MME |
| Synonyms | SCA, Neutral endopeptidase 24., CALLA, CD10, CMT2T, NEP, SCA43, SFE, MME, Atriopeptidase, Common acute lymphocytic leukemia antigen, Enkephalinase, Neprilysin, Neutral endopeptidase, Neutral endopeptidase 24.11, Skin fibroblast elastase, EPN, NEP, gp100, CD10 antigen, CD10), DKFZp686O16152, EC 3.4.24.11, enkephalinase), Membrane metallo endopeptidase, Membrane metallo endopeptidase (neutral endopeptidase, Membrane metallo endopeptidase variant 1, Membrane metallo endopeptidase variant 2, Membrane metalloendopeptidase, Membrane metalloendopeptidase neutral endopeptidase enkephalinase, Membrane metalloendopeptidase neutral endopeptidase enkephalinase CALLA CD10, Membrane metalloendopeptidase variant 1, Membrane metalloendopeptidase variant 2, membrane-associated, MGC126681, MGC126707, neprilysin-390, neprilysin-411 |
| Swissprot | |
| Calculated MW | 85 kDa |
| Observed MW |
95 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cell membrane |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Immunology, Stem Cells, Cancer |
| Clone No. | 6A10 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | This gene encodes a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). This protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. This gene, which encodes a 100-kD type II transmembrane glycoprotein, exists in a single copy of greater than 45 kb. The 5' untranslated region of this gene is alternatively spliced, resulting in four separate mRNA transcripts. The coding region is not affected by alternative splicing. |
Other Clones
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Unconjugated
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