CD117/c-kit Polyclonal Antibody(Capture/Detector) (AN004460P)
For research use only.
| Verified Samples |
Verified Samples in ELISA: Recombinant Mouse CD117/c-kit protein, Mouse serum, Mouse plasma, Human serum, Human plasma Verified Samples in WB: Mouse brain, Rat brain, Rat cerebellum |
| Dilution | ELISA Capture 2-8 μg/mL, ELISA Detector 0.1-0.4 μg/mL, WB 1:500-1:1000 |
| Isotype | Rabbit IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | ELISA Capture/Detector, WB |
| Clonality | Polyclonal |
| Immunogen | Recombinant Mouse CD117/c-kit Protein expressed by Mammalian |
| Abbre | CD117/c-kit |
| Synonyms | SOW, Gsfsow, SCO, W, Bs, Fdc, Ssm, SCO1, SCO5, SOW3, CD117, c-KIT, Tr-kit, Gsfsco1, Gsfsco5, Gsfsow3, Mast/stem cell growth factor receptor Kit, Proto-oncogene c-Kit, SCFR, Tyrosine-protein kinase Kit, Sl, Kit, SCFR, CD117, c-KIT, Fdc, Gsfsco1, Gsfsco5, Gsfsow3, SCO1, SCO5, SOW3, Ssm, Tr-kit, KIT proto-oncogene receptor tyrosine kinase, PBT |
| Swissprot | |
| Calculated MW | 110 kDa |
| Observed MW |
140 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Concentration | 1 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.2, containing 0.05% proclin 300. |
| Purification Method | Antigen Affinity Purification |
| Conjugation | Unconjugated |
| Storage | Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended. |
| background | The c-Kit proto-oncogene is the cellular homolog of the transforming gene of a feline retrovirus (v-Kit). The c-kit protein includes characteristics of a protein kinase transmembrane receptor. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
Other Clones
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Other Formats
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Unconjugated
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