For research use only.
| Verified Samples |
Verified Samples in WB: Jurkat Verified Samples in IHC: Human tonsil |
| Dilution | WB 1:500-1:2000, IHC 1:25-1:100 |
| Clonality | Polyclonal |
| Immunogen | Fusion protein of human CD1D |
| Abbre | CD1D |
| Synonyms | Antigen-presenting glycoprotein CD1d, CD1.1, CD1A, CD1D, CD1D antigen, CD1D antigen d polypeptide, CD1d, CD1d molecule, Cd1d1, HMC class I antigen like glycoprotein CD1D, Ly 38, MGC34622, R3, R3G1, T cell surface glycoprotein CD1d, Th, differentiation antigen CD1 alpha 3 |
| Swissprot | |
| Calculated MW | 38 kDa |
| Observed MW |
Refer to figures
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cell membrane. Endosome membrane. Lysosome membrane. Subject to intracellular trafficking between the cell membrane, endosomes and lysosomes. |
| Concentration | 0.7 mg/mL |
| Buffer | PBS with 0.05% NaN3 and 40% Glycerol,pH7.4 |
| Purification Method | Antigen affinity purification |
| Research Areas | Cancer, Immunology, Stem Cells |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | CD1d is a member of the CD1 family of transmembrane glycoproteins,which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. CD1d is an antigen-presenting protein that binds self and non-self glycolipids and presents them to T-cell receptors on NKT cells. When activated,NKT cells rapidly produce Th1 and Th2 cytokines. The molecular weight of unglycosylated CD1d is 38 kDa,while glycosylated form of CD1d is 48-55 kDa. |
Other Clones
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