CD20/MS4A1 Monoclonal Antibody (AN200196P)
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For research use only.
| Verified Samples |
Verified Samples in WB:?Raji, CD20 konckout Raji Verified Samples in IHC: Human tonsil, Human lymphoma Verified Samples in IP: Raji |
| Dilution | WB 1:500-1:2000, IHC-P 1:100-1:1000, IP 1-5 μL/mg of lysate |
| Isotype | IgG1 |
| Host | Mouse |
| Reactivity | Human |
| Applications | WB, IHC-P, IP |
| Clonality | Monoclonal |
| Immunogen | Recombinant Human CD20/MS4A1 Protein |
| Abbre | MS4A1 |
| Synonyms | LEU, CVID, B-lymphocyte antigen CD, Leukocyte surface antigen Leu, B-lymphocyte surface antigen B, Membrane-spanning 4-domains subfamily A member, MS4A1, B1, Bp35, CD20, CVID5, LEU-16, MS4A2, S7, B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1, Bp35, Ly-44, APY, ATOPY, B lymphocyte antigen CD20, B Lymphocyte Cell Surface Antigen B1, B-lymphocyte cell-surface antigen B1, Bp 35, CD 20, CD20 antigen, CD20 receptor, Fc epsilon receptor I beta chain, Fc Fragment of IgE high affinity I receptor for beta polypeptide, FCER1B, High affinity immunoglobulin epsilon receptor subunit beta, IgE Fc receptor subunit beta, IGEL, IGER, IGHER, LEU 16, LEU16, leukocyte surface antigen Leu 16, Ly44, Membrane spanning 4 domains A1, Membrane spanning 4 domains subfamily A member 2, MGC3969 |
| Swissprot | |
| Calculated MW | 33 kDa |
| Observed MW |
36 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Tissue Specificity | Expressed on B-cells. |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Immunology, Stem Cells, Cancer |
| Clone No. | A1048 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | CD20 is a 297 amino acid (aa) non-glycosylated type III membrane protein that is the founding member of the MS4A tetraspanin protein family. It is expressed on pre-B, naïve and mature B lymphocytes and B-cell lymphomas. CD20 is associated with lipid rafts upon crosslinking, and appears to play a role in regulation of ion influx. The one extracellular segment of 48 aa shows 65% aa sequence identity between human and mouse CD20. |
Other Clones
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