CD22 Polyclonal Antibody (D-AB-10188L)

For research use only.
Verified Samples |
Verified Samples in WB: Human Raji Verified Samples in IHC: Human tonsil |
Dilution | WB 1:500-1:1000, IHC 1:200-1:400 |
Isotype | IgG |
Host | Rabbit |
Reactivity | Human |
Applications | WB, IHC |
Clonality | Polyclonal |
Immunogen | Recombinant Human CD22 protein expressed by E.coli |
Abbre | CD22 |
Synonyms | B lymphocyte cell adhesion molecule, BL CAM, CD 22, CD22 antigen, CD22 molecule, Lectin 2, Leu14, Lyb8, MGC130020, SIGLEC 2, T cell surface antigen Leu 14, sialic acid binding Ig like lectin 2, B cell receptor CD22 precursor |
Swissprot | |
Calculated MW | 95 kDa |
Observed MW |
135 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
Concentration | 1 mg/mL |
Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
Purification Method | Antigen Affinity Purification |
Research Areas | Cancer, Immunology, Stem Cells |
Conjugation | Unconjugated |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
background | CD22,also known as Siglec-2 (sialic acid binding Ig-like lectin 2) or BL-CAM (B-lymphocyte cell adhesion molecule),is a 130-140 kDa,B-cell restricted,type I transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. The expression of CD22 is developmentally regulated. It is expressed at low levels in the cytoplasm of pro-B and pre-B cells and present on the cell surface only at mature stages of B-cell differentiation. Cell surface expression is lost during terminal differentiation into plasma cell and after B-cell activation. CD22 is an inhibitory receptor for B-cell receptor (BCR) signalling,preferentially binds to alpha-2,6-linked sialic acid and mediates B-cell B-cell interactions. It plays a crucial role in activation and differentiation of the B-cell. |
Other Clones
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Unconjugated
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