CD284 Polyclonal Antibody (E-AB-70375)
For research use only.
| Verified Samples |
Verified Samples in WB: Jurkat, RAW264.7, A20, Mouse spleen, Mouse lung, Mouse thymus, Rat spleen, Rat lung, Rat thymus Verified Samples in IHC: Mouse spleen, Rat lymph |
| Dilution | WB 1:500-1:2000, IHC 1:500-1:2000 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse TLR4. |
| Abbre | CD284 |
| Synonyms | ARMD10, CD284, CD284 antigen, Homolog of Drosophila toll, TLR 4, TLR4, TOLL, Toll like receptor 4, Toll-like receptor 4, hToll |
| Swissprot | |
| Calculated MW | 95 kDa |
| Observed MW |
95 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Membrane. |
| Concentration | 0.9 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 1% protein protectant and 50% glycerol. |
| Purification Method | Affinity purification |
| Research Areas | Cancer, Cardiovascular, Immunology, Microbiology |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | TLR4,also named as CD284,belongs to the Toll-like receptor family. TLR4 cooperates with LY96 and CD14 to mediates the innate immune response to bacterial lipopolysaccharide (LPS). TLR4 acts via MYD88,TIRAP and TRAF6,leading to NF-kappa-B activation,cytokine secretion and the inflammatory response. Three alternatively spliced transcript variants that encode different protein isoforms have been described. |
Other Clones
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Other Formats
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Unconjugated
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