CD45 Monoclonal Antibody (E-AB-22009)

For research use only.
Verified Samples |
Verified Samples in WB: Hela Verified Samples in IHC: Human lung cancer Verified Samples in IF: Human liver cancer |
Dilution | WB 1:500-1:2000, IHC 1:50-300 |
Isotype | IgG |
Host | Mouse |
Reactivity | Human |
Applications | WB, IHC-p |
Clonality | Monoclonal |
Immunogen | Synthetic Peptide |
Abbre | CD45 |
Synonyms | B220, CD 45, CD45, CD45 antigen, CD45R, GP180, L-CA, LCA, LY5, Leukocyte common antigen, Ly-5, Ly5, Lyt-4, OTTHUMP00000033813, OTTHUMP00000033816, OTTHUMP00000033817, OTTHUMP00000038574, Protein tyros, Protein tyrosine phosphatase receptor type c polypeptide, homolog of, loc |
Swissprot | |
Calculated MW | 147 kDa |
Observed MW |
147 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
Cellular Localization | Membrane |
Concentration | 1 mg/mL |
Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 0.5% protein protectant and 50% glycerol. |
Purification Method | Protein A purification |
Research Areas | Cancer, Cardiovascular, Immunology, Neuroscience, Signal Transduction, Stem Cells |
Clone No. | 3H3 |
Conjugation | Unconjugated |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
background | The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family.PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation.This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP.This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling.It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. |
Other Clones
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Other Formats
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Unconjugated
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