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For research use only.

Verified Samples Verified Samples in WB: Hela
Verified Samples in IHC: Human lung cancer
Verified Samples in IF: Human liver cancer
Dilution WB 1:500-1:2000,  IHC 1:50-300
Isotype IgG
Host Mouse
Reactivity Human
Applications WB,  IHC-p
Clonality Monoclonal
Immunogen Synthetic Peptide
Abbre CD45
Synonyms B220,  CD 45,  CD45,  CD45 antigen,  CD45R,  GP180,  L-CA,  LCA,  LY5,  Leukocyte common antigen,  Ly-5,  Ly5,  Lyt-4,  OTTHUMP00000033813,  OTTHUMP00000033816,  OTTHUMP00000033817,  OTTHUMP00000038574,  Protein tyros,  Protein tyrosine phosphatase receptor type c polypeptide,  homolog of,  loc
Swissprot
Calculated MW 147 kDa
Observed MW 147 kDa

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Membrane
Concentration 1 mg/mL
Buffer Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 0.5% protein protectant and 50% glycerol.
Purification Method Protein A purification
Research Areas Cancer,  Cardiovascular,  Immunology,  Neuroscience,  Signal Transduction,  Stem Cells
Clone No. 3H3
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.
background The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family.PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation.This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP.This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling.It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling.
Other Clones

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Unconjugated

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