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100μL $ 260.00
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For research use only.

Verified Samples Verified Samples in WB: Mouse colon
Verified Samples in IHC: Human lung cancer, Human stomach cancer, Human ovarian cancer
Verified Samples in IF: Human Hep G2
Dilution WB 1:200-1:500,  IF 1:50-1:200,  IHC 1:250-1:500
Isotype IgG
Host Rabbit
Reactivity Human,  Mouse
Applications WB,  IHC,  IF
Clonality Polyclonal
Immunogen Recombinant Mouse Ceacam1 protein expressed by E.coli
Abbre CEACAM1
Synonyms Antigen CD66,  BGP,  BGP 1,  BGP-1,  BGPI,  Biliary glycoprotein,  Biliary glycoprotein 1,  Biliary glycoprotein adhesion molecule,  Carcinoembryonic antigen related cell adhesion molecule 1,  carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein)
Swissprot
Calculated MW 57 kDa
Observed MW 110-160kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Concentration 1 mg/mL
Buffer PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4
Purification Method Antigen Affinity Purification
Research Areas Tags and Cell markers
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.
background This gene encodes a member of the carcinoembryonic antigen (CEA) gene family, which belongs to the immunoglobulin superfamily. Two subgroups of the CEA family, the CEA cell adhesion molecules and the pregnancy-specific glycoproteins, are located within a 1.2 Mb cluster on the long arm of chromosome 19. Eleven pseudogenes of the CEA cell adhesion molecule subgroup are also found in the cluster. The encoded protein was originally described in bile ducts of liver as biliary glycoprotein. Subsequently, it was found to be a cell-cell adhesion molecule detected on leukocytes, epithelia, and endothelia. The encoded protein mediates cell adhesion via homophilic as well as heterophilic binding to other proteins of the subgroup.
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Unconjugated

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