Cell Ferrous (Fe2+) Fluorometric Assay Kit (E-BC-F108)
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For research use only.
| Detection Principle |
"Studies have confirmed that iron is the most abundant transition metal element in living organisms, and it is involved in a variety of physiological activities. In recent years, free iron in cells have attracted more attention due to their high reactivity and their correlation with cell damage and death. Free iron exist in the cell as stable Fe2+ and Fe3+. Considering the reducing environment, metal transporter and water solubility of Fe2+, the behavior of Fe2+ is more important than that of Fe3+. This kit provides a fluorescent probe that can specifically bind to Fe2+, and the probe can enter the cell interior well, which is suitable for the detection of Fe2+ in alive cells. When the probe reacts with Fe2+, an irreversible orange (red) fluorescent product is generated (excitation wavelength: 530 nm emission wavelength: 580 nm)." |
| Sample Type | Cell |
| Detection Method | Fluorometric method |
| Detection Instrument | Fluorescence microplate reader, Fluorescence microscope, Flow cytometry |
| Research Area | Ferroptosis , Inorganic Ions |
| Other Reagents Required | PBS(0.01 M，pH = 7.4) or phenol red-free basal medium |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Assay Time | 40 min |
| Cat.No. | Product Name | Clone No. |
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