Cell Mitochondrial Complex Ⅲ (Coenzyme Q-Cytochrome C Reductase) Activity Assay Kit (E-BC-K836-M)
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For research use only.
| Detection Principle | As an organelle, mitochondria is the "power factory" in cells and the main site of aerobic respiration of cells. Its function is to convert energy through oxidative phosphorylation to provide energy for cellular activities. The oxidation process is carried out by four respiratory chain membrane protein complexes (complexes I, II, III and IV) on the inner mitochondrial membrane. Mitochondrial complex III, also known as cytochrome c reductase complex, its main function is to oxidize the reduced coenzyme Q10 formed by mitochondrial complexes I and II to oxidative coenzyme Q10. In this process, the OD value increased at 550 nm. Therefore, the activity of mitochondrial complex III can be quantified by measure the change OD value at 550 nm. |
| Synonyms | Coenzyme Q-Cytochrome C Reductase |
| Sample Type | Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (540-560 nm, optimum wavelength: 550 nm) |
| Research Area | Mitochondria Function , Energy Metabolism |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.73 U/L |
| Detection Range | 0.73-29.3 U/L |
| Precision | inter-assay CV: 4.5-10.0% | intra-assay CV: 3.5-5.0% |
| Sample Volume | 40 μL(cell homogenate) |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 1 × 10^6 Jurkat cells | 1 |
| 1 × 10^6 Hela cells | 1 |
| 4 × 10^6 K562 cells | 1 |
| 3 × 10^6 4T1 cells | 1 |
| 6 × 10^6 CHO cells | 1 |
| 1 × 10^6 293T cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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