Cell Mitochondrial Complex II Activity Assay Kit (E-BC-K835-M)
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For research use only.
| Detection Principle | Mitochondrial complex II, also known as succinate-coenzyme Q reductase, is widely found in the mitochondria of animals, plants, microorganisms and cultured cells. It catalyzes the oxidation of succinic acid in the TCA cycle to fumaric acid, which converts the ubiquinone to a reduced form through the basic unit structures of the complex such as iron-sulfur proteins. Coenzyme Q, the catalytic product of mitochondrial complex II, can further reduce 2,6-dichloroindoxol, which has a characteristic absorption peak at 600 nm. Therefore, the activity of mitochondrial complex II can be quantified by measure the change OD value at 600 nm. |
| Synonyms | succinate-coenzyme Q reductase |
| Sample Type | Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (590-610 nm, optimum wavelength: 600 nm) |
| Research Area | Mitochondria Function , Energy Metabolism |
| Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.54 U/L |
| Detection Range | 0.54-17.66 U/L |
| Precision | inter-assay CV: 3.0-6.0% | intra-assay CV: 2.0-5.0% |
| Sample Volume | 20 μL(cell homogenate) |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| HL-60 cell(1 × 10^6) | 1 |
| Hela cell(1 × 10^6) | 1 |
| CHO cell(4 × 10^6) | 1 |
| Jurkat cell(1 × 10^6) | 1 |
| K562 cell(1 × 10^6) | 1 |
| 293T cell(1 × 10^6) | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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