For research use only.
| Verified Samples |
Verified Samples in WB: various cell lines Verified Samples in IF: HT-29 |
| Dilution | WB 1:500-1:2000, IF 1:50-1:200 |
| Clonality | Polyclonal |
| Immunogen | Recombinant fusion protein of human CFTR |
| Synonyms | ABC35, ABCC7, CF, CFTR, CFTR/MRP, MRP7, TNR-CFTR, dJ760C5.1 |
| Swissprot | |
| Calculated MW | 69 kDa/161 kDa/168 kDa |
| Observed MW |
168 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cell membrane, Early endosome membrane, Multi-pass membrane protein. |
| Concentration | 1 mg/mL |
| Buffer | PBS with 0.05% proclin300,50% glycerol,pH7.3. |
| Purification Method | Affinity purification |
| Research Areas | Cancer, Cell Biology, Metabolism |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | This gene encodes a member of the ATP-binding cassette (ABC) transporter superfamily. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily that is involved in multi-drug resistance. The encoded protein functions as a chloride channel and controls the regulation of other transport pathways. Mutations in this gene are associated with the autosomal recessive disorders cystic fibrosis and congenital bilateral aplasia of the vas deferens. Alternatively spliced transcript variants have been described, many of which result from mutations in this gene. |
Other Clones
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Unconjugated
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