Cholinesterase (CHE) Activity Assay Kit (E-BC-K052-S)
For research use only.
| Detection Principle | Cholinesterase breaks down acetylcholine into choline and acetic acid. Acetylcholine that is not hydrolyzed by cholinesterase reacts with basic hydroxylamine to form acetamidamine. It reacts in an acidic solution to form a brown-red hydroxamate iron complex. The color depth is directly proportional to the amount of remaining acetylcholine, which can be colorimetrically quantified. Cholinesterase activity was calculated. |
| Synonyms | CHE |
| Sample Type | Whole blood,Serum,Plasma,Tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Spectrophotometer (520 nm) |
| Research Area | Liver And Renal Function |
| Other Reagents Required | Normal saline (0.9% NaCl) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 1.17 U/mL |
| Detection Range | 1.17-160 U/mL |
| Precision | inter-assay CV: 9.4% | intra-assay CV: 3.7% |
| Sample Volume | 0.05-0.1mL |
| Assay Time | 1 h |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 2-3 |
| Human plasma | 2-3 |
| Mouse serum | 2-3 |
| Mouse plasma | 2-3 |
| SH-SY5Y cells | 1 |
| 10% Mouse brain tissue homogenate | 1 |
| 10% Rat brain tissue homogenate | 1 |
| 10% Rat kidney tissue homogenate | 1 |
| 10% Rat spleen tissue homogenate | 1 |
| 10% Mouse liver tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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