Citric Acid (CA) Colorimetric Assay Kit (E-BC-K351-M)
For research use only.
| Detection Principle | In acidic condition, Cr (Ⅵ) will be reduced to Cr3+, Cr3+ reacts with citric acid. And the product has a characteristic absorption peak at 545 nm, therefore the content of citric acid in sample can be calculated by measuring the absorbance value at 545 nm. |
| Synonyms | CA |
| Sample Type | Serum,Plasma,Animal tissue,Mitochondria |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (535-555 nm, optimum wavelength: 545 nm) |
| Research Area | Mitochondria Function , Tricarboxylic Acid（TCA）Cycle , Energy Metabolism |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.06 mmol/L |
| Detection Range | 0.06-2.0 mmol/L |
| Precision | inter-assay CV: 0.04 | intra-assay CV: 0.04 |
| Sample Volume | 0.1 mL |
| Assay Time | 50 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 5-20 |
| Dog serum | 5-10 |
| Rat serum | 10-20 |
| Horse serum | 5-10 |
| Mouse plasma | 5-20 |
| 10% Rat brain tissue homogenate | 5-10 |
| 10% Rat liver tissue homogenate | 5-20 |
| 10% Rat kidney tissue homogenate | 5-10 |
| 10% Rat lung tissue homogenate | 15-30 |
| 10% Mouse heart tissue homogenate | 5-20 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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