COL3A1 Monoclonal Antibody (E-AB-22071)
For research use only.
Verified Samples |
Verified Samples in WB: Hela Verified Samples in IHC: Human liver Verified Samples in IF: Mouse liver |
Dilution | WB 1:500-1:2000, IHC 1:50-300, IF 1:100-1:300 |
Isotype | IgG |
Host | Mouse |
Reactivity | Human, Mouse, Rat |
Applications | WB, IHC-p, IF |
Clonality | Monoclonal |
Immunogen | Synthetic Peptide |
Abbre | Collagen III |
Synonyms | Alpha 1 type III collagen, Alpha1 (III) collagen, CO3A1, COL 3A1, COL3A1, Collagen III alpha 1 chain precursor, Collagen III alpha 1 polypeptide, Collagen alpha 1(III) chain, Collagen alpha-1(III) chain, Collagen type III alpha 1 (Ehlers Danlos syndrome type IV au |
Swissprot | |
Calculated MW | 139 kDa |
Observed MW |
138 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
Cellular Localization | Secreted>extracellular space>extracellular matrix. |
Concentration | 1 mg/mL |
Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
Purification Method | Protein A purification |
Research Areas | Cancer, Signal Transduction, Stem Cells |
Clone No. | 5G2 |
Conjugation | Unconjugated |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
background | This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. |
Other Clones
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Other Formats
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Unconjugated
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