Creatine Kinase (CK) Activity Assay Kit (E-BC-K558-M)
For research use only.
| Detection Principle | Creatine kinase (CK, EC 2.7.3.2) catalyze creatine phosphate and ADP to produce creatine and ATP. Hexokinase catalyze creatine and glucose to produce glucose-6-phosphate. Glucose-6-phosphate dehydrogenase (G6P-DH，Glucose-6-phosphate dehydrogenase) catalyze glucose-6-phosphate and NADP+ to produce NADPH which have a maximum absorption peak at 340 nm. The CK activity can be calculated by measuring the OD values at 340 nm. |
| Sample Type | Serum,Plasma,Animal tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (320-360 nm, optimum wavelength: 340 nm) |
| Research Area | Liver And Renal Function |
| Other Reagents Required | Double distilled water, PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 6.16 U/L |
| Detection Range | 13.55-115.86 U/L |
| Precision | inter-assay CV: 3.4-9.6% | intra-assay CV: 2.5-7.4% |
| Sample Volume | 10 μL |
| Assay Time | 35 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Human plasma | 1 |
| Mouse serum | 1 |
| Rat serum | 1 |
| 10% Rat kidney tissue homogenate | 1 |
| 10% Rat brain tissue homogenate | 2-5 |
| 10% Rat liver tissue homogenate | 2-10 |
| 1 × 10^6 HepG2 cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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