Cyclooxygenase (COX) Activity Fluorometric Assay Kit (E-BC-F050)
For research use only.
| Detection Principle |
Cyclooxygenase (COX), also known as prostaglandin endoperoxide synthase (PTGS), is a bifunctional enzyme that exhibits both cyclooxygenase and catalase activities. The cyclooxygenase activity of COX can catalyze the conversion of arachidonic acid (AA) to prostaglandin G2 (PGG2), while the catalase activity of COX can convert prostaglandin G2 to prostaglandin H2 (PGH2). Arachidonic acid generates prostaglandins under the catalysis of cyclooxygenase, and under the action of cofactor (heme), cyclooxygenase activates peroxidase activity, converting fluorescent red into fluorescent substance. |
| Sample Type | Animal tissue |
| Detection Method | Fluorometric method |
| Detection Instrument | Fluorescence microplate reader (Ex/Em=535 nm/587 nm) |
| Research Area | Metabolic Diseases |
| Other Reagents Required | Normal saline (0.9% NaCl) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.03 U/L |
| Detection Range | 0.03-9.66 U/L |
| Precision | inter-assay CV: 5.0-7.0% | intra-assay CV: 3.5-5.0% |
| Sample Volume | 15 μL |
| Assay Time | 1 h 10 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse liver tissue homogenate | 1 |
| 10% Mouse kidney tissue homogenate | 1 |
| 10% Rat liver tissue homogenate | 1-2 |
| 10% Rat kidney tissue homogenate | 1-2 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
Other Clones
{{antibodyDetailsPage.numTotal}} Results
-
{{item.title}}
Citations ({{item.publications_count}}) Manual MSDS
Cat.No.：{{item.cat}}
{{index}} {{goods_show_value}}
Other Formats
{{formatDetailsPage.numTotal}} Results
-
{{item.title}}
Citations ({{item.publications_count}}) Manual MSDS
Cat.No.：{{item.cat}}
{{index}} {{goods_show_value}}
-
IF：{{item.impact}}
Journal：{{item.journal}} ({{item.year}})
DOI：{{item.doi}}Reactivity：{{item.species}}
Sample Type：{{item.organization}}
-
Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}}
