Cytochrome P450 2D6 (CYP2D6) Activity Fluorometric Assay Kit (E-BC-F023)
For research use only.
| Detection Principle |
Cytochrome P450 2D6 (CYP2D6) is a member of the cytochrome P450 enzyme family that is responsible for metabolizing approximately 25% of drugs in the human body and is primarily expressed in the liver. CYP2D6 has significant metabolic effects on many clinically important drugs, including antidepressants, antipsychotics, beta-blockers, and painkillers. It converts these drugs into more excretable metabolites through oxidation reactions. The key role of CYP2D6 in drug metabolism and the significant influence of individual differences make detecting the activity of this enzyme important for personalized medicine. The detection principle of this kit: the substrate is converted into a fluorescent substance after catalysis by CYP2D6, and the fluorescence value is detected at the excitation wavelength of 390 nm and the emission wavelength of 468 nm, and the enzyme activity is calculated by the fluorescence value of the standard product. |
| Sample Type | Liver microsome |
| Detection Method | Fluorometric method |
| Detection Instrument | Fluorescence microplate reader (Ex/Em=390 nm/468 nm) |
| Research Area | Metabolic Diseases , Liver And Renal Function |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.29 U/L |
| Detection Range | 0.29-4.42 U/L |
| Precision | inter-assay CV: 9.0-9.9% | intra-assay CV: 2.1-3.1% |
| Sample Volume | 20 μL(liver microsome homogenate) |
| Assay Time | 2 h 20 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse liver microsomes | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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