For research use only.
| Detection Principle | Cytochrome P450 reductase (CPR) is an important part of the cytochrome P450 (CYPs) system. After obtaining electrons from the electron donor, CPR transfers electrons to CYPs, which react with oxidized substrates and molecules to form reduced substrates and water.<br/> This kit uses the electron transfer characteristics of CPR to catalyze the substrate to generate chromophore, which has a maximum absorption at 450 nm. The activity of CPR can be calculated by measureing the OD value at 450 nm. |
| Sample Type | Tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (430-470 nm, optimum wavelength: 450 nm) |
| Research Area | Oxidative Stress |
| Other Reagents Required | Normal Saline (0.9% NaCl) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 9.11 U/L |
| Detection Range | 9.11-550 U/L |
| Precision | inter-assay CV: 3.2-3.9% | intra-assay CV: 2.8-3.8% |
| Assay Time | 45 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse liver tissue homogenate | 1 |
| 10% Mouse lung tissue homogenate | 1 |
| 10% Mouse kidney tissue homogenate | 1 |
| 10% Mouse heart tissue homogenate | 1 |
| 1 × 10^6 CHO cells | 1 |
| 1 × 10^6 Jurkat cells | 1 |
| 1 × 10^6 293T cells | 1 |
| 0.375 × 10^6 A549 cells | 1 |
| 0.35 × 10^6 Hela cells | 1 |
| 0.5 × 10^6 Molt-4 cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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