Dap3 Polyclonal Antibody (E-AB-15595)
For research use only.
| Verified Samples |
Verified Samples in WB: HepG2, Hela Verified Samples in IHC: Human brain |
| Dilution | WB 1:1000-1:3000, IHC 1:25-1:100 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human , Mouse |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Immunogen | Synthetic peptide of mouse Dap3 |
| Synonyms | 4921514D13Rik, DAP-3 |
| Swissprot | |
| Calculated MW | 45 kDa |
| Observed MW |
Refer to figures
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Mitochondrion, mitochondrial matrix, mitochondrial small ribosomal subunit, mitochondrion, Nucleus, nucleoplasm. |
| Concentration | 0.6 mg/mL |
| Buffer | PBS with 0.05% sodium azide and 50% glycerol, PH7.4 |
| Purification Method | Affinity purification |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | 28S ribosomal protein S29, mitochondrial is a protein that in humans is encoded by the DAP3 gene. Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. This protein potentially binds ATP/GTP and might be a functional partner of the mitoribosomal protein S27. Splice variants that differ in the 5' UTR have been found for this gene; both variants encode the same protein. Pseudogenes corresponding to this gene are found on chromosomes 1q and 2q. DAP3 has been shown to interact with FADD, TNFRSF10A, Glucocorticoid receptor and Heat shock protein 90kDa alpha (cytosolic), member A1. |
Other Clones
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Other Formats
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Unconjugated
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