DHVD3(1,25-Dihydroxyvitamin D3) ELISA Kit (E-EL-0016)

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal DHVD3. During the reaction, Universal DHVD3 in the sample or standard competes with a fixed amount of Universal DHVD3 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal DHVD3. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal DHVD3 in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Similar to other steroid hormones, 1, 25-dihydroxyvitamin D3 binds to specific nuclear receptors in target cells, enters the nucleus, and regulates the expression of related genes (such as calcin-binding protein genes, osteocalcin genes, etc.). It can also open calcium channels through signal transduction system, and play a rapid regulatory role in calcium and phosphorus metabolism. 1, 25-dihydroxyvitamin D3 promotes the absorption of calcium and phosphorus in the small intestine, affects the calcium metabolism of bone tissue, thereby maintaining the normal level of blood calcium and blood phosphorus, and promotes the calcification of bone and teeth.