Dihydroorotate Dehydrogenase (DHODH) Activity Fluorometric Assay Kit (E-BC-F140)
For research use only.
| Detection Principle | Dihydroorotate Dehydrogenase (DHODH) is a key enzyme in the de novo synthesis pathway of pyrimidine nucleotides. DHODH catalyzes the formation of orotic acid and reacts with a chromogenic agent to produce a fluorescent substance. The fluorescence intensity is positively correlated with the activity of DHODH. |
| Sample Type | Animal tissue,Cell |
| Detection Method | Fluorometric method |
| Detection Instrument | Fluorescence microplate reader (Ex/Em=340 nm/450 nm) |
| Research Area | Metabolic Diseases |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.008 U/L |
| Detection Range | 0.008-2.5 U/L |
| Precision | inter-assay CV: 6.6%-8.6% | intra-assay CV: 2.2%-5.9% |
| Sample Volume | 30 μL(tissue homogenate/cell homogenate) |
| Assay Time | 1 h 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 5% Mouse liver tissue homogenate | 1 |
| 5% Mouse kidney tissue homogenate | 1 |
| 5% Mouse spleen tissue homogenate | 1 |
| 1×10^6 Hela cells | 1 |
| 1×10^6 Jurkat cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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